Polyphenol oxidase overexpression in transgenic Populus enhances resistance to herbivory by forest tent caterpillar (Malacosoma disstria).
Identifieur interne : 004210 ( Main/Exploration ); précédent : 004209; suivant : 004211Polyphenol oxidase overexpression in transgenic Populus enhances resistance to herbivory by forest tent caterpillar (Malacosoma disstria).
Auteurs : Jiehua Wang [Canada] ; C Peter ConstabelSource :
- Planta [ 0032-0935 ] ; 2004.
Descripteurs français
- KwdFr :
- Animaux (MeSH), Catechol oxidase (génétique), Catechol oxidase (métabolisme), Croisements génétiques (MeSH), Extraits de plantes (isolement et purification), Extraits de plantes (métabolisme), Feuilles de plante (enzymologie), Feuilles de plante (parasitologie), Immunité innée (génétique), Lepidoptera (pathogénicité), Maladies des plantes (parasitologie), Populus (enzymologie), Végétaux génétiquement modifiés (enzymologie).
- MESH :
- enzymologie : Feuilles de plante, Populus, Végétaux génétiquement modifiés.
- génétique : Catechol oxidase, Immunité innée.
- isolement et purification : Extraits de plantes.
- métabolisme : Catechol oxidase, Extraits de plantes.
- parasitologie : Feuilles de plante, Maladies des plantes.
- pathogénicité : Lepidoptera.
- Animaux, Croisements génétiques.
English descriptors
- KwdEn :
- Animals (MeSH), Catechol Oxidase (genetics), Catechol Oxidase (metabolism), Crosses, Genetic (MeSH), Immunity, Innate (genetics), Lepidoptera (pathogenicity), Plant Diseases (parasitology), Plant Extracts (isolation & purification), Plant Extracts (metabolism), Plant Leaves (enzymology), Plant Leaves (parasitology), Plants, Genetically Modified (enzymology), Populus (enzymology).
- MESH :
- chemical , genetics : Catechol Oxidase.
- chemical , isolation & purification : Plant Extracts.
- chemical , metabolism : Catechol Oxidase, Plant Extracts.
- enzymology : Plant Leaves, Plants, Genetically Modified, Populus.
- genetics : Immunity, Innate.
- parasitology : Plant Diseases, Plant Leaves.
- pathogenicity : Lepidoptera.
- Animals, Crosses, Genetic.
Abstract
In order to functionally analyze the predicted defensive role of leaf polyphenol oxidase (PPO; EC 1.10.3.1) in Populus, transgenic hybrid aspen (Populus tremula x P. alba) plants overexpressing a hybrid poplar (Populus trichocarpa x P. deltoides) PtdPPO1 gene were constructed. Regenerated transgenic plants showed high PPO enzyme activity, PtdPPO1 mRNA levels and PPO protein accumulation. In leaf disk bioassays, forest tent caterpillar (Malacosoma disstria) larvae feeding on PPO-overexpressing transgenics experienced significantly higher mortality and reduced average weight gain compared to larvae feeding on control leaves. However, this effect was observed only when older egg masses were used and the resulting larvae showed reduced growth and vigor. In choice tests, no effect of PPO overexpression was detected. Although PPO in poplar leaves is latent and requires activation with detergents or trypsin for full enzymatic activity, in caterpillar frass the enzyme was extracted in the fully activated form. This activation correlated with partial proteolytic cleavage, suggesting that PPO latency and activation during digestion could be an adaptive and defense-related feature of poplar PPO.
DOI: 10.1007/s00425-004-1327-1
PubMed: 15309534
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Stn CSC, Victoria, BC, V8W 3N5, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Centre for Forest Biology and Department of Biology, University of Victoria, PO Box 3020, Stn CSC, Victoria, BC, V8W 3N5</wicri:regionArea><wicri:noRegion>V8W 3N5</wicri:noRegion></affiliation></author><author><name sortKey="Constabel, C Peter" sort="Constabel, C Peter" uniqKey="Constabel C" first="C Peter" last="Constabel">C Peter Constabel</name></author></analytic><series><title level="j">Planta</title><idno type="ISSN">0032-0935</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Catechol Oxidase (genetics)</term><term>Catechol Oxidase (metabolism)</term><term>Crosses, Genetic (MeSH)</term><term>Immunity, Innate (genetics)</term><term>Lepidoptera (pathogenicity)</term><term>Plant Diseases (parasitology)</term><term>Plant Extracts (isolation & purification)</term><term>Plant Extracts (metabolism)</term><term>Plant Leaves (enzymology)</term><term>Plant Leaves (parasitology)</term><term>Plants, Genetically Modified (enzymology)</term><term>Populus (enzymology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux (MeSH)</term><term>Catechol oxidase (génétique)</term><term>Catechol oxidase (métabolisme)</term><term>Croisements génétiques (MeSH)</term><term>Extraits de plantes (isolement et purification)</term><term>Extraits de plantes (métabolisme)</term><term>Feuilles de plante (enzymologie)</term><term>Feuilles de plante (parasitologie)</term><term>Immunité innée (génétique)</term><term>Lepidoptera (pathogénicité)</term><term>Maladies des plantes (parasitologie)</term><term>Populus (enzymologie)</term><term>Végétaux génétiquement modifiés (enzymologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Catechol Oxidase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Plant Extracts</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Catechol Oxidase</term><term>Plant Extracts</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Feuilles de plante</term><term>Populus</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Plant Leaves</term><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Immunity, Innate</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Catechol oxidase</term><term>Immunité innée</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Extraits de plantes</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Catechol oxidase</term><term>Extraits de plantes</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Feuilles de plante</term><term>Maladies des plantes</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Plant Diseases</term><term>Plant Leaves</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Lepidoptera</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Lepidoptera</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Crosses, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Croisements génétiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In order to functionally analyze the predicted defensive role of leaf polyphenol oxidase (PPO; EC 1.10.3.1) in Populus, transgenic hybrid aspen (Populus tremula x P. alba) plants overexpressing a hybrid poplar (Populus trichocarpa x P. deltoides) PtdPPO1 gene were constructed. Regenerated transgenic plants showed high PPO enzyme activity, PtdPPO1 mRNA levels and PPO protein accumulation. In leaf disk bioassays, forest tent caterpillar (Malacosoma disstria) larvae feeding on PPO-overexpressing transgenics experienced significantly higher mortality and reduced average weight gain compared to larvae feeding on control leaves. However, this effect was observed only when older egg masses were used and the resulting larvae showed reduced growth and vigor. In choice tests, no effect of PPO overexpression was detected. Although PPO in poplar leaves is latent and requires activation with detergents or trypsin for full enzymatic activity, in caterpillar frass the enzyme was extracted in the fully activated form. This activation correlated with partial proteolytic cleavage, suggesting that PPO latency and activation during digestion could be an adaptive and defense-related feature of poplar PPO.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15309534</PMID><DateCompleted><Year>2005</Year><Month>05</Month><Day>12</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0032-0935</ISSN><JournalIssue CitedMedium="Print"><Volume>220</Volume><Issue>1</Issue><PubDate><Year>2004</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Planta</Title><ISOAbbreviation>Planta</ISOAbbreviation></Journal><ArticleTitle>Polyphenol oxidase overexpression in transgenic Populus enhances resistance to herbivory by forest tent caterpillar (Malacosoma disstria).</ArticleTitle><Pagination><MedlinePgn>87-96</MedlinePgn></Pagination><Abstract><AbstractText>In order to functionally analyze the predicted defensive role of leaf polyphenol oxidase (PPO; EC 1.10.3.1) in Populus, transgenic hybrid aspen (Populus tremula x P. alba) plants overexpressing a hybrid poplar (Populus trichocarpa x P. deltoides) PtdPPO1 gene were constructed. Regenerated transgenic plants showed high PPO enzyme activity, PtdPPO1 mRNA levels and PPO protein accumulation. In leaf disk bioassays, forest tent caterpillar (Malacosoma disstria) larvae feeding on PPO-overexpressing transgenics experienced significantly higher mortality and reduced average weight gain compared to larvae feeding on control leaves. However, this effect was observed only when older egg masses were used and the resulting larvae showed reduced growth and vigor. In choice tests, no effect of PPO overexpression was detected. Although PPO in poplar leaves is latent and requires activation with detergents or trypsin for full enzymatic activity, in caterpillar frass the enzyme was extracted in the fully activated form. This activation correlated with partial proteolytic cleavage, suggesting that PPO latency and activation during digestion could be an adaptive and defense-related feature of poplar PPO.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Jiehua</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Centre for Forest Biology and Department of Biology, University of Victoria, PO Box 3020, Stn CSC, Victoria, BC, V8W 3N5, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Constabel</LastName><ForeName>C Peter</ForeName><Initials>CP</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate 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